ORIGINAL_ARTICLE
FINDING A DIFFERENT LENGTH OF MICROSATELLITE REPEATS IN THE COMPLETED GENOME OF PHOENIX DACTYLIFERA CHLOROPLAST
Variation of SSRs is mainly caused by slipped-strand mispairing and subsequent resulting errors during DNA replication, repair, and recombination. Regular expressions applied to match the repeats of DNA sequences. In this study a programmed tool was designed by Visual basic language to detect the different lengths of SSR sequences in the chloroplast completed genome of Phoenix dactylifera (Accession number, GU811709.2). The accession number GU811709.2was downloaded from Genbank of NCBI and used as an input string to search for the different lengths of repeats by using RFRE Ver. 1.0. Different lengths of repeats (Di-repeats, Tetra-repeats, Penta-repeats and hexa –repeats) were founded by the regular expression language which used by the RFRE tool. The length of the longest repeats was forty characters. Parts of the long repeats were founded in the region of expressed gene (rps3 gene). The most abundant founded motif was (AAAA) {2} and the total length of that repeat was equal to 632.
The tool was very easy to use where it had a GUI interface and accepted different combined regular expression syntax to match different patterns of repeats in the genome of Phoenix dactylifera (Accession number, GU811709.2).The founded patterns assigned to the annotated genome map of the accession numberGU811709.2. A specific Regular Expression Pattern ([agct]{20})\1 was used to find a repeated sequences with 40 characters in length in three different locations in the genome of chloroplast (8675, 78458 and 85021).
In conclusion, this study focused on the importance of characterized and identifying the types of tandem repeats in Phoenix dactylifera (cultivar, Khalas- female) chloroplast genome by using the power of regular expression language.
https://jpd.journals.ekb.eg/article_41885_d41d8cd98f00b204e9800998ecf8427e.pdf
2017-04-01
171
198
10.21608/jpd.2017.41885
Phoenix dactylifera
Repeater Finder Regular Expression
SSR
Tandem- repeats
Mohamed
Ezz
1
Bioinformatics Department, Genetic Engineering and Biotechnology Research Institute (GEBRI), SADAT City University, Egypt.
LEAD_AUTHOR
Amal
Hussein
2
Bioinformatics Department, Genetic Engineering and Biotechnology Research Institute (GEBRI), SADAT City University, Egypt.
AUTHOR
Alaa
Hemeida
3
Bioinformatics Department, Genetic Engineering and Biotechnology Research Institute (GEBRI), SADAT City University, Egypt.
AUTHOR
Fawzy
El-Feky
4
Biotechnology Department, Faculty of Agriculture, Al-Azhar University, Egypt.
AUTHOR
ORIGINAL_ARTICLE
TECHNOLOGICAL AND BIOLOGICAL STUDIES ON CAROTENOID IN CALENDULA AND BOUGAINVILLEA.
In this research, carotenoids pigments were extracted from petals, of both yellow bougainvillea and calendula by both petroleum ether and diethyl ether as organic solvents.
The organic solvent diethyl ether more efficient in extraction than petroleum ether, too. Petals of calendula contain more carotenoids than that in yellow bougainvillea. In biological experiment, the white rats were injected by different doses from carotene extracts from both yellow bougainvillea and calendula after 45 days from injection there is no any effect in liver function, kidney function and Rate of death these results sure its safety.
On the other hand there is a slight gradually decreased in the content of the extracted carotenoids in storage for 12weeks.
Conclusivevly,carotenoids pigments were extracted from petals of both yellow bougainvillea and calendula by both petroleum ether and diethyl ether from the obtained data, diethyl ether more efficient in extraction than petroleum ether too, petals ofcalendula contain more Carotenoids than that in yellow bougainvillea. The extracted pigments were safe biologically.
https://jpd.journals.ekb.eg/article_41892_d41d8cd98f00b204e9800998ecf8427e.pdf
2017-04-01
199
206
10.21608/jpd.2017.41892
Technological & Biological Studies
Carotenoid
Calendula
Bougainvillea
Mmdouh
Azzazy
1
Hort. Tech. Res. Dept., Food Tech. Inst., Agric. Res Center, Egypt,
LEAD_AUTHOR
Saad
Soliman
2
Hort. Tech. Res. Dept., Food Tech. Inst., Agric. Res Center, Egypt,
AUTHOR
Khaled
Mahroos
3
Poultry Department, Fac of Agric-Zagazig Univ., Egypt.
AUTHOR
ORIGINAL_ARTICLE
TECHNOLOGICAL AND BIOLOGICAL STUDIES ON RED PIGMENTS IN RED BOUGAINVILLEA AND MATHIOLA.
The natural pigments considered the more safety in public health as natural colorants in food instead of the harmful synthetic colorants in this study, red Anthocyanins pigments were extracted from red bougainvillea (Bougainvillea commerson ex) and mathiola (Matthiola incane) petals as a natural colorant by the following methods: (1) by using ethanolic solution acidified by Hcl. (2) by using Distilled water (3) by using Distilled water acidified by 0.1% citric acid. (4) by using Distilled water acidified by 0.3% citric acid. It could be noticed that the first method is the most effective in the extractions of the pigments but the last method is the most economically. On the other hand, in biological experiment, there is no any bad effect on the liver and the kidney functions and there is no any case of death after 45 days of injection of rats with these anthocyanins extracts. too, noticed that there is a gradually decrease of anthocyanins content by storage for 3months. Conclusively, anthocyanins pigments were extracted from red bougainvillea and mathiola petals as natural colorants of foods instead of the harmfull synthetic colorants. In biological experiments , it were safe which there is no any bad effect on the liver and the kidney functions and there is no any case of death during 45 days. Too, noticed that there is a slight gradually decrease of anthoyanins content by storage for 3 months.
https://jpd.journals.ekb.eg/article_41893_7eef522e28ef2bcbd626f984277c3343.pdf
2017-04-01
207
217
10.21608/jpd.2017.41893
Technological & Biological Studies
Red Pigments
Red Bougainvillea
Mathiola
Mmdouh
Azzazy
1
Hort. Tech. Res. Dept., Food Tech. Inst., Agric. Res. Center, Egypt.
LEAD_AUTHOR
Adlly
Abd El Sattar
2
Crop. Tech Res. Dept., Food Tech. Inst., Agric. Res. Center, Egypt
AUTHOR
Mohamed
Abd El Hak
3
Poultry Dept., Fac. Of Agric., Zagazig Univ. Egypt.
AUTHOR
ORIGINAL_ARTICLE
RESPONSE OF SWEET POTATO PLANT TO THE SAME SOURCES OF ORGANIC MANURE AND APPLICATION METHODS OF POTASSIUM HUMATE UNDER SANDY SOIL CONDITIONS.
Two field experiments were performed during two consecutive summer seasons of 2014 and 2015 at El-Kassasien Hort. Res. Station, Ismailia Governorate, Egypt, to study the effect of organic manure sources (recommended chemical fertilizers, FYM, compost and chicken manure) and potassium humate ( without, soil application and foliar application) at the rate of 2 g/L ,as well as, different combination between them on plant growth, chemical constituents, yield and its components and storage root quality of sweet potato (Ipomoea batatas, L.) cv. Abees grown under sandy soil condition with drip irrigation system.
Application of organic manure fertilizers caused significant increases in growth parameter, yield and its components and mineral contents compared to control treatment (without organic fertilization). meanwhile, application of chicken manure at the rate of 5 m3/fed. achieved highest values of all aforementioned parameters, followed by the application of compost at the rate of 15 m3/fed and FYM at the rate of 15 m3/fed, in a descending order, respectively.
Spraying sweet potato plants with potassium humate significantly enhanced plant growth, yield and its components and mineral contents (NPK content in tuber root TSS, reducing and total sugar as well as total carbohydrate contents). In this respect, spraying plants with 2 g/L potassium humate attained the superiority impacts comparing the other treatments.
Hence, it can be concluded that fertilizing sweet potato plants by chicken manure at the rate of 5 m3/fed. in combined with spraying plants by potassium humate at the rate of2 g/L gave the highest values of growth, yield and its components and chemical constituents as compared to other interaction treatments.
Conclusively, this study showed that the highest yield of sweet potato storage roots and storage roots quality were obtained from chicken manure at 5 m3/fed., foliar application was potassium humate (2 g/L). The interaction between 5 m3 chicken manure and potassium humate as foliar application at the rate of2 g/L.
https://jpd.journals.ekb.eg/article_41895_3ab09765b5bbf2f2af7c0962656306af.pdf
2017-04-01
219
240
10.21608/jpd.2017.41895
Sweet potato
organic manure
potassium humate
Growth
yield and chemical constituents
Abrahim
Al Esaily
1
Hort. Research Institute, Agricultural Research Center, Giza, Egypt.
LEAD_AUTHOR
ORIGINAL_ARTICLE
THE ECONOMIC IMPACTS OF REACHING THE OPTIMUM AND THE MOST PROFITABLE SIZE FOR THE SUMMER ORANGE PRODUCERS
This research aimed to study the production and economic efficiency for the summer orange crop and the economic impacts of reaching the optimum and the most profitable size for the producers. The research depended on the economic and the descriptive and quantitative analysis, and the field data for random sample which consisted of 120 farmers in 2015/2016.
The results indicated that the fixed production costs represented 355 from the total costs, the variable costs represented 65.5%, from which, the human labor 16.42%, the chemical fertilizers 19.66%, the measure fertilizers 6.675, the machinery work 6.675, crop transport 5.9%, the transplants 5%, and the pest cides and insecticides 4.665% from the total production costs per feddan.
The results also, indicated that summer orange production is far from realizing the optimum and the most profitable size. The actual average production per feddan was about 14.75 ton, the average price was 1100 L.E/ton the total production value was 16225 L.E/ feddan. While, the optimum production size was 17.23 ton, with average costs 455.88 l.e /ton. The economic production was 20.13 ton, which exceeds the actual production by 5.38 ton.
The results indicated that there was a positive relationships between the productivity per feedan and the number of transplants, and the quantity of manure fertilizer, and the quantity of phosphoric fertilizers, and the number of hours by machinery work, and the total human work, and the nitrogenous fertilizer, as independent variables. The total production elasticity estimated by 0.745 which represent decreasing return to scale.
The economic impacts of reaching the optimum and the most profitable size of summer orange include increase in the total revenue, and the net return by feddan, and increase the return and the profit for the producer and Sharkia Governorate.
https://jpd.journals.ekb.eg/article_41896_e04f256d1009b7013d053f62c35251cf.pdf
2017-04-01
241
265
10.21608/jpd.2017.41896
economic impacts
profitable size
summer orange producers
Ahmed
Hamed
1
Agric. Econ. Dept., Fac. Agric., Zagazig Univ., Egypt
LEAD_AUTHOR
ORIGINAL_ARTICLE
EFFECT OF FOLIAR SPRAYING ROYAL JELLY ON GROWTH AND FRUITING OF ZEBDA MANGO TREES GROWN UNDER ASWAN REGION CONDITIONS
During 2015 and 2016 seasons, Zebda mango trees treated once (at the beginning of growth), twice (at growth the beginning and again just after fruit setting), or thrice (at growth start, just after fruit setting and at one month later) with Royal Jelly at 0.0125 up to 0.05 %. The effects of Royal jelly treatments on growth and fruiting of the trees were investigated.
Treating the trees once, twice, or thrice with Royal Jelly at 0.0125 up to 0.05% caused a remarkable promotion on all growth characteristics, leaf pigments and various nutrients, yield as well as both physical and chemical characteristics of the fruits compared with the control treatment. There was a gradual promotion on growth traits, nutrients, yield and fruit quality with increasing concentrations and frequencies of application of Royal Jelly.The differences between the two higher concentrations (0.025 & 0.05%) and frequencies (twice & thrice) as promotion on these parameters were insignificantly effected in both seasons.
Conclusively, the best results with regard to yield and fruit quality of Zebda mango trees grown under Aswan region conditions were obtained when the trees sprayed twice at growth start and again just after fruit setting with Royal Jelly at 0.025%.
https://jpd.journals.ekb.eg/article_41897_beaffbe68fab3f81c07756a3f52a67f6.pdf
2017-04-01
267
285
10.21608/jpd.2017.41897
royal jelly
yield
fruit quality
Zebda mangoes
Mohamed
El- Sayed
faissalfadel@yahoo.com
1
Hort. Dept. Fac. of Agric. Minia Univ., Egypt
LEAD_AUTHOR
Ahmed
Mohamed
2
Tropical Fruits Res. Dept. Hort. Res. Instit., ARC, Giza, Egypt
AUTHOR
ORIGINAL_ARTICLE
EFFECT OF SOME ORGANIC N FERTILIZERS ENRICHED WITH SPIRULINA PLATENSIS ALGAE AS A PARTIAL REPLACEMENT OF INORGANIC N FERTILIZER ON GRWOTH AND FRUITING OF SUPERIOR GRAPEVINES
During 2014, 2015 and 2016 seasons, Superior grapevines were fertilized with three organic manures (poultry manure, plant compost and farmyard manure) enriched with Spirulina platensis algae as a partial replacement of 25 to 75% inorganic N. The merit was detecting the best source of organic N enriched with Spirulina platensis algae applied with inorganic N that responsible for producing higher yield with better quality berries.
Replacing 50% of inorganic N by 50% organic manures enriched with Spirulina platensis algae at 20 ml/vine enhanced growth, yield and cluster weight while replacing 75% of inorganic N by 75% organic manures enriched with Spirulina platensis algae at 40 ml/vine gave favourable effects on vine nutritional status and quality of the grapes. Shot berries percentage was greatly declined by N as 25% inorganic N plus 75% organic manures enriched with Spirulina platensis algae at 40 ml/vine. The best organic manures in this respect was poultry manure, plant compost and farmyard manure, in descending order.
Conclusively, for enhancing growth and yield of Superior grapevines, it is suggested to supply the vines with N (60g/vine/year) through 50% inorganic N + 50% poultry manure enriched with Spirulina platensis algae at 20 ml/vine/year. Fertilizing the vines with N as 25% inorganic N + 75% poultry manure enriched with 40 ml Spirulina platensis algae gave the best results with regard to berries quality.
https://jpd.journals.ekb.eg/article_41898_50d94c1ba35657c936fb7e36c366588c.pdf
2017-04-01
287
306
10.21608/jpd.2017.41898
organic N
Inorganic N
Spirulina platensis algae
Superior grapevines
Asmaa
Ebrahim
1
Viticulture Res. Dept. Hort. Res. Instit. ARC, Giza, Egypt.
LEAD_AUTHOR
Mahmoud
Gad El-Kareem
2
Hort. Dept., Fac. of Agric., Souhag Univ., Egypt.
AUTHOR
ORIGINAL_ARTICLE
RESPONSE OF SUPERIOR GRAPEVINES TO FOLIAR SPRAYSELENIUM WITH SOME VITAMINS
This study was carried out during 2015 and 2016 seasons to investigate the effect of different concentrations and frequencies of application (once, twice or thrice) of selenium and some vitamins (B complex, A and D) on growth, vine nutritional status, yield and berries quality of Superior grapes.
The treatment of grapevine once, twice or thrice with a mixture of selenium at 25 to 100 ppm as well as vitamins B complex (B1 + B2 + B6 + B12) at 250 to 1000 ppm and vitamins A and D at 25 to 100 ppm caused a remarkable promotion on all growth aspects, pigments and nutrients in the leaf, yield as well as both physical and chemical characteristics of the berries over the check treatment.
Conclusively, subjecting superior grapevines grown under Minia and the resembling conditions twice with a mixture of Selenium at 50 ppm in combined with vitamins B complex (B1+B2+B6+B12) at 500 ppm, A and D each at 50 ppm was responsible for improving yield and berries quality.
https://jpd.journals.ekb.eg/article_41899_3813ac3500dd42b73742c02591f94689.pdf
2017-04-01
307
321
10.21608/jpd.2017.41899
Superior grapevines
selenium
Vitamins B
A and D
Growth
yield
berries quality
Moawad
Mohamed
1
Hort. Dept. Fac. of Agric. Minia Univ., Egypt.
LEAD_AUTHOR
Hamdy
Mahmoud
2
Hort. Dept. Fac. of Agric. Minia Univ., Egypt.
AUTHOR
Hassan
Abo El-Fadle
3
Hort. Dept. Fac. of Agric. Minia Univ., Egypt.
AUTHOR
Mohamed
Abada
4
Viticulture Res. Dept. Hort. Institute, ARC. Giza, Egypt.
AUTHOR
ORIGINAL_ARTICLE
EFFECT OF SOME PLANT EXTRACTS ON GROWTH AND NUTRITIONAL STATUS OF FLAME SEEDLESS GRAPEVINE TRANSPLANTS
A pot experiment was conducted during 2014 and 2015 seasons to test the efficacy of eleven plant extracts (seven oils of garlic, onion, peppermint, olive, clove, flax seed and anise plus four extracts of turmeric, green tea, moringa leaf and red chillies) each at 5% on growth and nutritional status of Flame seedless grapevine transplants.
Results showed that the previous eleven plant extracts were very effective in enhancing all growth aspects (plant height, stem thickness, number of leaves/plant, number of lateral shoots/plant, main shoot length, number of leaves/shoot, leaf area and dry weight of whole plant), root parameters (root distribution area, number of secondary roots per main root, main root length and dry weight of roots/plant), leaf pigments (chlorophylls a &b, total chlorophylls and total carotenoids), percentages of total carbohydrates and soluble sugars in the leaves and uptake of N, P and K by transplant over the check treatment. The best plant extracts in this respect could be arranged as follows in descending order garlic oil, turmeric extract, onion oil, green tea extract, moringa leaf extract, red chillies extract, peppermint oil, clove oil, olive oil, flax seed oil and anise oil.
Conclusively, for producing vigorous flame seedless grapevine transplants, it is necessary to spray the one-year old transplant with garlic oil or turmeric extract each at 5% four times at the middle of April, May, June and July yearly.
https://jpd.journals.ekb.eg/article_41900_13e2bb32d512442f1bad096cfb314f98.pdf
2017-04-01
323
337
10.21608/jpd.2017.41900
Flame seedless grapevine transplants
Plant extracts
Growth
vine nutritional status
Ahmed
Akl
1
Hort. Dept. Fac. of Agric. Minia Univ. Egypt.
LEAD_AUTHOR
Fatma
Gadalla
2
Hort. Dept. Fac. of Agric. Minia Univ. Egypt.
AUTHOR
Mohamed
Abada
3
Viticulture Res. Dept. Hort. Instit. ARC. Giza, Egypt
AUTHOR
ORIGINAL_ARTICLE
EVALUATION OF GROWTH AND FRUITING OF SOME OLIVE CVS GROWN UNDER MINIA REGION CONDITIONS
Seven olive cvs Aggezi Akse, Aggezi Shami, Picual, Frantoio, Coratina, Superinso and Koroneiki grown under Minia conditions were evaluated for their growth, flowering, yield and fruit quality during 2015 and 2016 seasons.
An obvious variation on all the investigated growth, leaf content of pigments and nutrients, flowering, yield and fruit characteristics were observed among the seven olive cvs. Olive cvs Aggezi Akse, Aggezi Shami and Picual exhibited the highest values of these parameters. Olive cv Koroneiki had the lowest values.
According to the values of yield and fruit characteristics during both seasons, it is suggested to plant olive cvs Aggezi Akse, Aggezi Shami and Picual, respectively under Minia region conditions.
Conclusively, based on relatively higher yield and good fruit quality, it is suggested to select olive cv. Aggezi Akse, Aggezi Shami, Picual, Frantoio, Coratina, Superinso and Koroneiki in descending order to plant under Siwa Oasis conditions.
https://jpd.journals.ekb.eg/article_41901_f229a3258fd189d2830ce3a3d9dafeb2.pdf
2017-04-01
339
353
10.21608/jpd.2017.41901
Evaluation
olive cvs
Growth
flowering
yield and fruit characteristics
Moawad
Mohamed
1
Hort. Dept., Fac. of Agric., Minia Univ., Egypt.
LEAD_AUTHOR
Aly
Gabra
2
Hort. Dept., Fac. of Agric., Minia Univ., Egypt.
AUTHOR
Awad
Fakhry
3
Hort. Dept., Fac. of Agric., Minia Univ., Egypt.
AUTHOR
Mahmoud
El-Kareem
4
Hort. Dept., Fac. of Agric., Souhag Univ., Egypt
AUTHOR
ORIGINAL_ARTICLE
EFFECTIVENESS OF USING GUIDES FOR AGRICULTURAL INFORMATION SOURCES IN SHARKYIA AND DAKAHLIA GOVERNORATES
The aim of this research is to identify some of the personal characteristics of the agricultural extension workers, determine the degree of efficiency of the agricultural extension agents in the use of various sources of information (oral, written or electronic), and determine the factors affecting the efficiency of the agricultural extension agents in the use of various sources of information, whether oral, written or electronic. To identify the most important obstacles faced by agricultural extension agents in the use of different sources of information and the most important proposals to overcome them, to identify the problems faced by agricultural extension workers and their proposals for solving them
The study was conducted in the governorates of Al-Sharkiya and Dakahlia. Two centers were selected from each governorate: Mit Ghamr Center, Sinbillawin Center, Dakahlia Governorate, Husseiniya Center and Zagazig Center, Sharkiya Governorate. 50 guides were selected from each center. The sample size was 200 agricultural guides. Governorate, and data was collected using the questionnaire form by interview.
The results showed a significant correlation between the efficiency of guides in the use of verbal information sources and between age, work experience in agricultural extension, number of training courses, job satisfaction, motivation for achievement, feeling of community development and the direction of coordination between agricultural extension and other organizations.
The adequacy of the number of workers in the place, the qualifications of the workers, and the awareness of the workers for agricultural extension objectives
The results also showed a significant correlation between the efficiency of the guides in the use of written information resources and age, work experience in agricultural extension, number of training courses, job satisfaction, motivation for achievement, feeling of community development, And the adequacy of the number of workers in the field, the qualifications of the workers, and the awareness of the workers for agricultural extension objectives). The results also revealed a significant correlation between the efficiency of the guides in the use of electronic sources of information.
The number of training courses, job satisfaction, motivation for achievement, the sense of community development, the direction of coordination between agricultural extension and other organizations, the adequacy of the number of workers in the field, the qualifications of workers and the awareness of workers for agricultural extension purposes), while the relationship was age- , And did not confirm the statistical significance of the relationship with the sense of belonging to the community.
https://jpd.journals.ekb.eg/article_41903_1f7dcca1252f912e80ecb61d75fda669.pdf
2017-04-01
355
376
10.21608/jpd.2017.41903
Using Guides
Agricultural Information Sources
Sharkyia & Dakahlia Governorates
Shaimaa
Basiony
1
Agricultural Extension, Faculty of Agriculture, Zagazig University, Zagazig, Egypt.
LEAD_AUTHOR
ORIGINAL_ARTICLE
A COMPARATIVE STUDY OF THE COMPREHENSIVE QUALITY STANDARDS OF ACCREDITED AND NONACCREDITED RURAL HEALTH UNITS FROM HEALTH MINISTRY IN A VARIANT DEVELOPMENT LEVEL VILLAGES IN SHARKIA GOVERNORATE, EGYPT
The core objective of this Research is to explore the main variables influencing on the accreditation of Rural Health Units in Sharkia governorate. The field study was conducted in the most advanced and the less advanced villages in Sharkia Governorate on the rural health units, data were collected by a questionnaire by face to face structured interviews with a simple random sample of (71) of managers of rural health units (20% from 354 units), 34 accredited rural health units (17 units from the most advanced villages and 17 units from the less advanced villages), and 37 nonaccredited rural health units (18 units from the most advanced villages and 19 units from the less advanced villages).
These data were collected during the period from the beginning of September to the end of November 2015. Binary logistic regression forward (conditional) results showed that only four standards from fifteen standards had positive significant influences on the variable accreditation the rural health units, these standards together explained 75% of total variance (quality of firm management 61.7%, quality of Infection control clinical safety 4.5%, quality of quality improvement programme 7.4%, and quality of information management 1.4%).
https://jpd.journals.ekb.eg/article_41904_e1f7768a1c28345614d9ce2c4f695e7b.pdf
2017-04-01
377
408
10.21608/jpd.2017.41904
Comprehensive Quality Standards
Accredited and Nonaccredited Rural Health Units
Egypt
Ayman
Ecresh
1
Branch of Rural Sociology and Agric. Exten., Agric. Econ. Dept., Fac. Agric., Zagazig Univ., Egypt
LEAD_AUTHOR
Hazem
Seliem
2
Branch of Rural Sociology and Agric. Exten., Agric. Econ. Dept., Fac. Agric., Zagazig Univ., Egypt
AUTHOR