ORIGINAL_ARTICLE
EFFECT OF APPLICATION METHODS AND CONCENTATION OF CHITOSAN ON GROWTH, YIELD, TUBER ROOTS QUALITY AND STORABILITY OF SWEET POTATO PLANTS GROWN UNDER SANDY SOIL CONDITIONS
This work was carried out during the two successive summer seasons of 2013 and 2014 at a Private Vegetable Farm in El-Khattara distract, Sharkia, Governorate, to investigate the effect of different application methods and concentrations of chitosan ( 0, 0.025, 0.075 and 0.125 %) on growth , yield , tuber roots quality and storability of sweet potato cv. Buregard grown under sandy soil conditions using drip irrigation system. Dipping the base of the stem cuttings in chitosan solution before planting + foliar spraying of the plants with chitosan was the best method for increasing dry weight of shoots, N,P and K uptake by shoots, marketable yield and total yield and also, for decreasing weight loss (%) and decay (%) in tuber roots during storage period. Chitosan solution 0.075 % increased growth, average tuber weight , total yield, starch and total sugar in tuber roots, whereas chitosan 0.125 % increased chlorophyll a, total (a+b) in leaf tissues, N,P and K uptake by shoots and marketable yield and also recorded minimum values of weight loss and decay (%) in tuber roots during storage period. Dipping the base of the stem cuttings in 0.075 % chitosan solution before planting + spraying plants with 0.075 % chitosan solution gave the highest values of vine length , dry weight of shoots/ plant, chlorophyll a and total chlorophyll ( a+b) concentration in leaf tissues, average weight of tuber root, total yield , whereas the same treatment gave the lowest values of weight loss percentage and decay (%) in tuber roots during storage period. On the other hand, the base of stem cuttings in 0.125 % chitosan solution before planting + spraying plants with 0.125 % chitosan gave the highest values of N,P and K uptake, marketable yield, starch and total sugar contents in tuber roots. Conclusively, from the foregoing results of this study, it could be concluded that, under the same conditions, dipping the base of the stem cutting in 0.075 % chitosan solution before planting + spraying plants with 0.075 % chitosan solution three times at 15 days intervals beginning 25 days after transplanting gave the best interaction treatment for increasing plant growth, yield and its components, tuber roots quality and storability of sweet potato plants.
https://jpd.journals.ekb.eg/article_42670_0977e73e21758917193e0e6bf82f0b5c.pdf
2015-08-01
237
261
10.21608/jpd.2015.42670
Sweet potato
Chitosan
stem cutting
foliar application
yield and storability
Elsayied
Abou El-Khair
1
Potato and Vegetatively Propagated Vegetables Res. Dept., Hort. Res. Inst. Agriculture Research Center, Giza, Egypt.
AUTHOR
ORIGINAL_ARTICLE
ISOLATION AND EXPRESSION OF DEHYDRIN AND DREB2 TRANSCRIPTION FACTOR GENES IN SOME WHEAT (Triticum asetivum L. ) CULTIVARS.
Drought is a worldwide problem, constraining global crop production and quality seriously. Water drives agricultural production in many parts of the world. Two genes from Triticum asetivum, were isolated under drought conditions.These drought responsive genes selected for expressional analyses can be classified into two groups. DHN gene with length 360 bp belong to the first group includes functional proteins already known to be involved in the response to water. DREB2 transcription factor gene with length 1256 bp belong to the second group that comprises protein factors involved in the regulation of signal transduction and gene expression.
https://jpd.journals.ekb.eg/article_42797_2c2c58145ec5be534b11aceb8a973941.pdf
2015-08-01
263
271
10.21608/jpd.2015.42797
Isolation & Expression
Dehydrin & Dreb2 Transcription Factor Genes
Triticum asetivum L. Cultivars
Ahmed
Salah
1
Molecular Biology Department, Genetic Engineering and Biotechnology Research Institute, GEBRI, Sadat city University, Egypt.
LEAD_AUTHOR
Amal
Abd El-Aziz
2
Molecular Biology Department, Genetic Engineering and Biotechnology Research Institute, GEBRI, Sadat city University, Egypt.
AUTHOR
el sayed
El-Absawy
3
Bioinformatics Dept., GEBRI, Sadat city University, Egypt.
AUTHOR
Mohamed
Nasr
4
Molecular Biology Department, Genetic Engineering and Biotechnology Research Institute, GEBRI, Sadat city University, Egypt.
AUTHOR
ORIGINAL_ARTICLE
DEVELOPMENT OF HAPLOID PLANTS THROUGH ANTHER CULTURE TECHNIQUE IN WHEAT ( Triticum aestivum L. )
Anther culture was utilized to develop haploid plants in wheat .This study was carried out at Plant Biotechnology Department, Genetic Engineering and Biotechnology Research Institute ( G E B R I ) from 2009 to 2014 years . Three varieties of wheat were used , Sids1, Gemmeiza 9 and Giza 168. The studied factors were, cold and chemical pretreatments, growth regulators concentrations of 2,4-D and kinetin and effect of source of spikes . The results indicated that the cold pretreatment had no effect on anther response of three wheat varieties, the chemical pretreatments by sterilization substances (ethanol alcohol 70 % or mercurec chloride 1g /L or thiourea 2 g /L ) were very important and essential to induce callus from anther . The three treatments of ( 1mg /L 2,4-D ) , ( 2 mg /L 2,4-D ) and ( 2 mg /L 2,4-D + 0.1 mg /L kinetin ) gave the highest response of callus induction and plant regeneration . Androgenesis improved with increasing the cultured area which the spikes were collected from it .
https://jpd.journals.ekb.eg/article_42802_dab1edc5608e3aa3e127398773da4679.pdf
2015-08-01
285
296
10.21608/jpd.2015.42802
Wheat
Anther culture
Cold & chemical pretreatments
growth regulators
Effect of source of spikes
varieties
Haron
Abo Shama
1
Plant Biotech Dept . Genet. Engin. and Biotech .Res. Inst ( GEBRI), Sadat City, University Menufiya, Egypt.
LEAD_AUTHOR
Hala
Ghareeb
2
Plant Biotech Dept . Genet. Engin. and Biotech .Res. Inst ( GEBRI), Sadat City, University Menufiya, Egypt.
AUTHOR
ORIGINAL_ARTICLE
DEVELOPMENT OF HAPLOID PLANTS THROUGH OVARY CULTURE TECHNIQUE IN WHEAT ( Triticum aestivum L ).
This research was done at department of Plant Biotechnology, Genetic Engineering and Biotechnology Research Institute( GEBRI ), Sadat City university, Menufyia, Egypt from 2009 to 2014 years to study the effect of four factors affected callus induction of ovary cultur i.e.(cold pretreatment, chemical pretreatments, growth regulators and spikes sources. and establishment a technique to produce haploid plants. Three wheat varieties were used, Sids 1, Gemmeiza 9 and Giza 168. The results indicated that, chemical pretreatments by sterilization substances (Ethanol alcohol 70% or Mercurec chloride 1g/L or Thiourea 2g/L) were very important and essential to induce calli from ovaries. The combination between (1 and 2 mg /L 2,4-D ) and (0 , 0.1 and 0.2 mg/L kinetien) gave the highest response of callus induction and plant regeneration percentages. The estimates were increased and developed with increasing the cultured area which the spiks were collected from it. The estimates were varied with varieties.
https://jpd.journals.ekb.eg/article_42806_55257b9a9cd5778bbb489cbef88d620e.pdf
2015-08-01
297
306
10.21608/jpd.2015.42806
Wheat
Ovary culture
Cold & chemical pretreatments
growth regulators
Source of spikes
varieties
Haron
Abo Shama
1
Plant Biotech. Department Genetic Engineering and Biotech. Research Institute ( GEBRI ), Sadat City. Univ. Menufyia, Egypt
LEAD_AUTHOR
Hala
Ghareeb
2
Plant Biotech. Department Genetic Engineering and Biotech. Research Institute ( GEBRI ), Sadat City. Univ. Menufyia, Egypt.
AUTHOR
yasin
yasin
3
Plant Biotech. Department Genetic Engineering and Biotech. Research Institute ( GEBRI ), Sadat City. Univ. Menufyia, Egypt.
AUTHOR
ORIGINAL_ARTICLE
EFFECT OF FOLIAR SPRAY WITH SOME NATURAL PLANT EXTRACTS ON EARLINESS, PRODUCTIVITY AND QUALITY OF GLOBE ARTICHOKE
This study was conducted to estimate whether foliar spray with some natural plant extracts could enhance earliness, productivity and quality of globe artichoke hence, two field experiments were carried out during two seasons of 2012 /2013 and 2013/ 2014 at the Hort. Res. Farm of El-Bramoon, Dakahlia Governorate, to investigate the effect of foliar spray with garlic extract rates (1, 2 and 3 ml/L) and moringia leaf extract rates (1, 2 and 3 ml/L) on earliness, productivity and quality as well as chemical constituents of globe artichoke(Cynara scolymus L.) plants cv. Balady.
The main results can be summarized as follows:
The results indicated that increasing moringia leaf extract and garlic extract rates up to 3 ml/L of each were accompanied with significant increases in vegetative growth characters (plant height, Leaf area of leaves, dry weight of leaves and number of shoots/plant) and yield distribution (early, middle , late yield and total yield/fed.) compared with control.
Application of moringia leaf extract and garlic extract rates improved most head quality characteristics (head weight, length, diameter, receptacle fresh and dry matter). However, there were no significant differences in terms of head TSS in the second seasons.
Dry matter, N, P, K, protein, total sugars and total carbohydrates contents in receptacle were positively and significantly responded to moringia leaf extract and garlic extract rates in the two seasons.
The foliar spraying with moringia leaf extract at rates of 3 ml/L seemed to be the most effective treatment, which gave a balanced vegetative growth, a higher early yield as well as a good quality.
Moreover, this particular treatment was more beneficial compared to all other treatments.
https://jpd.journals.ekb.eg/article_42809_40899909b342bd69db059bc4c580c182.pdf
2015-08-01
307
324
10.21608/jpd.2015.42809
Cynara scolymus L
garlic extract
moringa extract
Usama
Saif Eldeen
1
Hort. Research Institute , Agric. Research Center (ARC), Giza, Egypt.
LEAD_AUTHOR
ORIGINAL_ARTICLE
EFFECT OF BORON, COPPER AND HUMIC ACID TREATMENTS ON VEGETATIVE GROWTH, YIELD AND STORABILITY OF JERUSALEM ARTICHOKE TUBERS
These experiments were carried out during two successive summer seasons of 2013 and 2014 on Jerusalem artichoke cv. Feusa. The plants were grown at Kaha Vegetables Res. Farm, Hort. Res. Inst., Agric. Res. Centre, under clay loam soil with using surface irrigation system, to study the effect of boron , copper and humic acid on vegetative growth, yield and its components , chemical constituents and storability of Jerusalem artichoke tubers. The experiment including 9 treatments ( foliar spray with 70 and 140 ppm boron, 50 and 100 ppm copper, 0.5 and 1 g/l humic acid as foliar application and 0.5 and 1 g/l humic acid as soil application beside of the control treatment ( spraying with water). The experiment was arranged in a randomized complete block design with three replicates.
The obtained results showed that the highest value of plant height was recorded by the plants which for sprayed with 50 or 100 ppm copper . While the plants which sprayed with 140 ppm boron gave the highest number of main stems per plant. Application of humic acid 1 g/l as soil application increased dry weight of foliage, number of tubers, yield per plant and total yield per feddan. Moreover, sprayed plants with 140 ppm boron treated plants with 1 g/l humic acid as soil application produced more average tuber weight than the other tested treatments. Chemical composition (N, P, K, Total carbohydrates and inulin contents) of Jerusalem artichoke was significantly affected with application all treatments than control treatment.The results also confirmed that weight loss and decay percentage in Jerusalem artichoke tubers increased with prolongation of storage period.
In addition, the plants, which sprayed with copper or boron in both concentrations, gave the lowest values in weight loss and decay percentage in Jerusalem artichoke tubers during the storage period.
Conclusively, from the obtained data in this study, it can be recommended by using humic acid at soil for improve growth, number of tubers, yield per plant and total yield. On the other hand, foliar application of boron on Jerusalem artichoke plants increased average tuber weight. Also, foliar spray with copper or boron helps to storage the tubers and low rate of weight loss and decay.
https://jpd.journals.ekb.eg/article_42813_c9fc5baef4411c6578befd261cc4ab6f.pdf
2015-08-01
325
342
10.21608/jpd.2015.42813
Jerusalem artichoke
Boron
copper
Humic acid
storability
weight loss and decay percentage
Mahmoud
Samy
1
Potato and Vegetatively Propagated Vegetables Department, Hort. Res., Institute, Egypt.
LEAD_AUTHOR
Nagwa
Mohamed
2
Potato and Vegetatively Propagated Vegetables Department, Hort. Res., Institute, Egypt.
AUTHOR
Mervat
Abd El-Aziz
3
Potato and Vegetatively Propagated Vegetables Department, Hort. Res., Institute, Egypt.
AUTHOR
ORIGINAL_ARTICLE
LOWER THRESHOLD TEMPERATURE AND THERMAL UNIT OF AMERICAN BOLLWORM, Helicoverpa armigera (HÜBNER) REARED ON PEA AND LETTUCE AND ITS REARING ON A NEW MODIFIED ARTIFICIAL DIETS
The present work was conducted to study the relationship between developmental stages of the cotton bollworm, Helicoverpa armigera (Hübner) (ABW) reared in the laboratory on pea and lettuce and the required thermal units (DD's ) at constant temperatures, 23, 27 and 30°C ± 1°C. Also, to evaluate the use of two new modified artificial diets (A and B) for mass rearing of ABW and compared with natural food (lettuce). Diets A and B contained dried active yeast, ascorbic acid, sorbic acid, methyl parahydroxy benzoate, liquid milk, vitamin mixture and formaldehyde 34 - 38%. In addition to the essential ingredient, in diet A is kidney beans and wheat grated while, diet B is chickpea and wheat grated.
Results showed that there was variation in developmental periods of the ABW in different stages reared on pea and lettuce. Temperature increasing led to an increase in the developmental rate and reduce the development periods of the different stages. The generation period of the ABW reared on lettuce was shorter (32.23 days) than that reared on pea (34.70 days). The lower developmental temperatures were 11.60 and 11.39 °C for the generation and the thermal units required were 467.13 and 511.26 DD's, respectively.
On the other hand, results showed that highest larval and pupal weight, adult emergence and number of eggs/female (560 eggs/female) and lowest larval and pupal mortality percentage of ABW were observed on diet B followed by diet A and lastly natural food (lettuce).
In conclusion, the two new modified artificial diets (A and B) developed in this study has proved to be satisfactory for mass rearing of ABW, Helicoverpa armigera (Hübner).
https://jpd.journals.ekb.eg/article_43592_f2e70037debe8319d39f3a0bb12d8232.pdf
2015-08-01
273
284
10.21608/jpd.2015.43592
lower threshold temperature & thermal unit
American bollworm
Helicoverpa armigera
pea & lettuce
new modified artificial diets
Adel
Amr
1
Plant Protection Research Institute, Agricultural Research Center, Dokki, Egypt.
AUTHOR
Aly
ELSAYED
2
Plant Protection Research Institute, Agricultural Research Center, Dokki, Egypt.
AUTHOR