MEAN PERFORMANCE, GENETIC DIVERSITY AND DNA ANALYSIS IN M3 GENERATION OF SOME WHEAT MUTANTS UNDER WATER STRESS

Document Type : Original Article

Authors

1 Plant Production, Faculty of Technology and Development, Zagazig University Egypt.

2 Plant Production, Faculty of Technology and Development, Zagazig University Egypt

3 Cell Research Institute. Agricultural Research Center, Giza., Egypt.

Abstract

The field experiments was conducted during the growing season of 2019/2020, to study the mean performance and genetic diversity using RAPD Marker in M3 generation of some wheat mutants with influenced by gamma ray and sodium azaide under water stress conditions, at the Experimental Farm, Faculty of Technology and Development, Zagazig University, Egypt. The all recommended cultural practices for wheat production except irrigation. It was irrigated immediately after sowing the first irrigation were applied after 45 days from sowing and the second irrigation up to the flowering stage. The experimental design was complete blocks design with three replicates. The analysis of variance showed a significant differences among Gimmieza-11 and there M3 mutants for days to heading, spike length, number of spikelets/spike, number of spikes/plant, number of grains/spike, spike grain weight/g, 1000-grain weight/g, grain yield/plant, biological yield/plant and harvest index. The  all mutants from Gemmeiza-11 were give the highest values of  mean performance for grain yield plant, The Sids-12 0.08 sodium azide (SA) had the highest value (45.39) followed by Sids-12 350Gy3 (40.74) and Sids-12 350 Gy1 (39.87) thy were more than control (38.99) for harvest index %. RAPD data analysis showed that, primers applied on wheat genotypes produced 18 bands; 16 of these were polymorphic. Primer OPB-5 gave the highest number of bands (5) and primers O-7, A-20 and OPB-7 had the lowest number (3).The highest percentage of polymorphic bands were produced by primers O-7, A-6 and OPB-5 (100%) and the lowest percentage of polymorphic bands were produced by A-20 and OPB-7 (67%).
Conclusively, this study established the importance of gamma rays and sodium azide  mutagenesis increating genetic variation within and among wheat breeding populations. wide phenotypic variation in mutants under each breeding population were identified for improving drought tolerance yield, and yield-related traits.
 

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