Abd-Allah, E., E. Z. Eliraqy, E., Salem, M. (2019). EFFECT OF ADDITION L-ARGININE TO EXTENDER OF POOR MOTILE HOLSTEIN BULLS SPERMATOZOA.. Journal of Productivity and Development, 24(1), 165-179. doi: 10.21608/jpd.2019.41328
Emad Abd-Allah; Entsar E. Z. Eliraqy; Mogda Salem. "EFFECT OF ADDITION L-ARGININE TO EXTENDER OF POOR MOTILE HOLSTEIN BULLS SPERMATOZOA.". Journal of Productivity and Development, 24, 1, 2019, 165-179. doi: 10.21608/jpd.2019.41328
Abd-Allah, E., E. Z. Eliraqy, E., Salem, M. (2019). 'EFFECT OF ADDITION L-ARGININE TO EXTENDER OF POOR MOTILE HOLSTEIN BULLS SPERMATOZOA.', Journal of Productivity and Development, 24(1), pp. 165-179. doi: 10.21608/jpd.2019.41328
Abd-Allah, E., E. Z. Eliraqy, E., Salem, M. EFFECT OF ADDITION L-ARGININE TO EXTENDER OF POOR MOTILE HOLSTEIN BULLS SPERMATOZOA.. Journal of Productivity and Development, 2019; 24(1): 165-179. doi: 10.21608/jpd.2019.41328
EFFECT OF ADDITION L-ARGININE TO EXTENDER OF POOR MOTILE HOLSTEIN BULLS SPERMATOZOA.
1Biotechnology Res. Dep. Animal Production Res. Institute, Agric. Res. Center, Egypt.
2Animal Production Res. Institute, Agric. Res. Center, Egypt
Abstract
This study was carried out to investigate the effect of addition of different levels of L-arginine (0, 3, 5, 7 and 10 mM) to improve the poor motile of Holstein bulls spermatozoa. Semen collected from five Holstein bulls once weekly and ejaculates with 45-50% mass motility was pooled. Semen was diluted with Tris- egg yolk extender at 37C° and divided into five parts,1st part without supplement (control), other four parts 2nd , 3rd , 4th and 5th were supplemented with 3, 5, 7 and 10 mM L-arginine respectively. Diluted semen was equilibrated at 4 C° for 4 h, then, filled in 0.25 ml French straws. Straws were kept on liquid nitrogen vapors for 10 min and then plunged and stored in liquid nitrogen at -196C°. Progressive sperm motility, live sperm, abnormal sperm and sperm acrosome integrity were determined post-dilution, equilibration and post-thawing processing. The results were referred that extenders with concentrations of 5 and 7mM L-Arginine were improved all parameters studied than 3; 10 mM L-arginine extenders and control extender at different cryopreservation processes. The percentage of DNA damage were 20.0, 10.8, 4.40, 3.20 and 9.40 for control and 3, 5, 7 and 10 mM L-arginine extenders respectively. Significant differences in chromatin integrity were observed among control and treatments (P Conclusively, it could be concluded that adding L-arginine with concentrations of 5 or 7mM to Tris-extender improved the freezability and fertility of poor quality of Holstein bull spermatozoa.
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