EFFECT OF ADDITION L-ARGININE TO EXTENDER OF POOR MOTILE HOLSTEIN BULLS SPERMATOZOA.

Abd-Allah, Emad; E. Z. Eliraqy, Entsar; Salem, Mogda

doi:10.21608/jpd.2019.41328

EFFECT OF ADDITION L-ARGININE TO EXTENDER OF POOR MOTILE HOLSTEIN BULLS SPERMATOZOA.

Document Type : Original Article

Authors

¹ Biotechnology Res. Dep. Animal Production Res. Institute, Agric. Res. Center, Egypt.

² Animal Production Res. Institute, Agric. Res. Center, Egypt

10.21608/jpd.2019.41328

Abstract

This study was carried out to investigate the effect of addition of different levels of L-arginine (0, 3, 5, 7 and 10 mM) to improve the poor motile of Holstein bulls spermatozoa. Semen collected from five Holstein bulls once weekly and ejaculates with 45-50% mass motility was pooled. Semen was diluted with Tris- egg yolk extender at 37C° and divided into five parts,1^st part without supplement (control), other four parts 2^nd , 3^rd , 4^th and 5^th were supplemented with 3, 5, 7 and 10 mM L-arginine respectively. Diluted semen was equilibrated at 4 C° for 4 h, then, filled in 0.25 ml French straws. Straws were kept on liquid nitrogen vapors for 10 min and then plunged and stored in liquid nitrogen at -196C°. Progressive sperm motility, live sperm, abnormal sperm and sperm acrosome integrity were determined post-dilution, equilibration and post-thawing processing.
The results were referred that extenders with concentrations of 5 and 7mM L-Arginine were improved all parameters studied than 3; 10 mM L-arginine extenders and control extender at different cryopreservation processes. The percentage of DNA damage were 20.0, 10.8, 4.40, 3.20 and 9.40 for control and 3, 5, 7 and 10 mM L-arginine extenders respectively. Significant differences in chromatin integrity were observed among control and treatments (P
Conclusively, it could be concluded that adding L-arginine with concentrations of 5 or 7mM to Tris-extender improved the freezability and fertility of poor quality of Holstein bull spermatozoa.

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