INDUCTION OF SPAWNING IN AFRICAN CATFISH (Clarias gariepinus ) BY USING HUMAN CHORIONIC GONADOTROPIN

Mahsoub, Fatma; El Gaafay, Mohamed; Rashwan, Aly; Akr, Adel

doi:10.21608/jpd.2019.42099

INDUCTION OF SPAWNING IN AFRICAN CATFISH (Clarias gariepinus ) BY USING HUMAN CHORIONIC GONADOTROPIN

Document Type : Original Article

Authors

¹ Department of Animal & Poultry Production, Faculty of Technology and Development, Zagazig University, Zagazig Egypt.

² Department of Fish Hatching and Reproductive Physiology, Central Laboratory for Aquaculture Research (CLAR), Abbassa Abo- Hammad, Sharkia, Egypt .

10.21608/jpd.2019.42099

Abstract

This work was designed to study the effect of Human chorionic gonadotropin (HCG) on induction of spawning of African catfish (Clarias gariepinus ). In this experiment a total number of 90 catfish (745.8±9.2g body weight) were divided into three experimental groups (30 each) .The first group was considered as a control group and injected intramusculary with carp pituitary extract (0.3ml/kg/ b.w.).The second group was injected with HCG (1200 IU / kg body weight ). The third group was injected with both carp pituitary extract and HCG (0.15 ml/kg and 600 IU/kg body weight, respectively.
The results of the present experiment showed significant differences in survival rate , fertilization rate (%), hatching rate (%), latency period, egg weight (g), egg diameter (mm) and total protein (g/dl). However, insignificant differences were detected in initial weight (g), condition factor and specific growth rate, body weight before spawning, body weight after spawning, egg number/g, gonadosomatic index, hepatosomatic index, cortisol (ug/dl) and estradiol17β(pg/m). Females treated with HCG had the highest values of fertilization rate, hatching rate, latency time and survival rate.
Conclusively, the results of the present study indicated that at a level of 1200 IU from HCG was the most effective agent in inducing spawning of African catfish (Clarias gariepinus) females.

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