Ibrahim, I., Emara, H., El-Banna, A., Shams El-Din, I. (2010). In-vitro CULTIVATION OF Drosera capensis AS AN INSECTIVOROUS PLANTS AND ITS EXTRACT EFFECT ON LARVA OF RED PALM WEEVIL. Journal of Productivity and Development, 15(2), 237-250. doi: 10.21608/jpd.2010.42414
Ibrahim Ibrahim; Hamdy Emara; Abd el-Moneam El-Banna; Ibrahim Shams El-Din. "In-vitro CULTIVATION OF Drosera capensis AS AN INSECTIVOROUS PLANTS AND ITS EXTRACT EFFECT ON LARVA OF RED PALM WEEVIL". Journal of Productivity and Development, 15, 2, 2010, 237-250. doi: 10.21608/jpd.2010.42414
Ibrahim, I., Emara, H., El-Banna, A., Shams El-Din, I. (2010). 'In-vitro CULTIVATION OF Drosera capensis AS AN INSECTIVOROUS PLANTS AND ITS EXTRACT EFFECT ON LARVA OF RED PALM WEEVIL', Journal of Productivity and Development, 15(2), pp. 237-250. doi: 10.21608/jpd.2010.42414
Ibrahim, I., Emara, H., El-Banna, A., Shams El-Din, I. In-vitro CULTIVATION OF Drosera capensis AS AN INSECTIVOROUS PLANTS AND ITS EXTRACT EFFECT ON LARVA OF RED PALM WEEVIL. Journal of Productivity and Development, 2010; 15(2): 237-250. doi: 10.21608/jpd.2010.42414
In-vitro CULTIVATION OF Drosera capensis AS AN INSECTIVOROUS PLANTS AND ITS EXTRACT EFFECT ON LARVA OF RED PALM WEEVIL
1Plant Biotechnology Department, Genetic Engineering and Biotechnology Research Institute (GEBRI), Minufiya University, Egypt.
2Central Laboratory of Date Palm Research and Development, Agricul-tural Research Center, Giza, Egypt.
Abstract
Carnivorous or insectivorous plants belong to several botanical families, the most important of them is Droseraceae, which includes Drosera plants. Insecticide substances are extracted from Drosera. Tissue culture technique provides the best way to obtain as high and clean quantity as possible of the biomass needed to obtain these substances. This study aimed to propagate the Drosera capensis in-vitro and studying the effect of Drosera capensis residue on larva of red palm weevil. Shoots were visible on leaf explants, apparently forming directly on leaf surfaces without intermediate callus. The best results of shoot number (13.8 shoots per explant) and length (2.93 cm) were obtained at 0.05 mg L-1 BA compared with the control, BA-free media, observed 2.8 shoots per explant and 2.27 cm in length. Shoots were sub-cultured on half strength of MS medium supplemented with four concentrations of IBA (0, 0.5, 1.0, 2.0 mg L-1) in rooting stage. MS basal medium supplemented with 1.0 mg L-1 IBA achieved the best root formation where the root number was 47.3 per plant. The residue of Drosera capensis plants at different concentrations (0.0, 50.0, 100. 500.0 mg per liter) had been given to fully developed larvae of red palm weevil (Rhynchophorus ferrugineus Oliv.) through their feeding diet. Larvae were obtained from the field and were maintained on the stems of sugarcane prior to mass rearing, artificial diet, which was formulated from sucrose, molasses, potatoes and agar. The residue of Drosera capensis had toxicological effects on R. ferrugineus larvae. The lethal action of Drosera capensis residue had appeared clearly at 500 mg L-1 where the lethal percentage of red palm weevil larva was 65% after ten days.
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