MOLECULAR IDENTIFICATION OF TOMATO GERMPLASM FOR RESISTANCE TO TOMATO YELLOW LEAF CURL VIRUS IN EGYPT

Document Type : Original Article

Authors

1 Horticulture Research Institute, Agricultural Research Center, Cairo, Egypt.

2 Soil, Water and Environment Research Institute,Giza, Egypt

Abstract

The present investigation was carried out to study molecular marker are known to confer resistance to tomato yellow leaf curl virus (TYLCV) in some local and hybrid  Fourteen tomato accessions namely,  Jubilee, Peto86,Super Strain B,Advantag, Edkawi, super Bader, Tempo, Azteca, G.5, G.S, Nada, 9062, 9065, 9064. The plants were grown in under greenhouse conditions, open field and test for reactions to TYLCV.
The resistance to TYLCV disease in the tomato accession linked to Ty-2 gene. All the plants in the studs one band only 800 bp were susceptible to the disease; expat 9064 two bands 800bp and 900 bp were resistant.
Conclusively, the use of methods of molecular markers to identify resistant varieties and sensitive to viruses. Moreover, the use of modern molecular methods to identify the local plant varieties with jam and before inclusion in breeding programs to save time and effort and money.
       

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