IMPROVED MICRO PROPAGATION OF Cichorium intybus L. FROM LEAF EXPLANT

Shahin, Heba; Yasseen, Yasseen Mohamed; Fahim, Mohamed

doi:10.21608/jpd.2015.42778

IMPROVED MICRO PROPAGATION OF Cichorium intybus L. FROM LEAF EXPLANT

Document Type : Original Article

Authors

¹ Genetic Engineering and Biotechnology Research Institute (GEBRI), Sadat City University, , P.O. Box 79/22857, Egypt.

² Genetic Engineering and Biotechnology Research Institute (GEBRI), Sadat City University, Sadat City, P.O. Box 79/22857, Egypt.

10.21608/jpd.2015.42778

Abstract

Cichorium intybus is edible, medicinal and forage plant. The pharmaceutical raw materials were obtained from wild chicory (var. Maurane). Currently, farmers are increasingly assuming plantations of wild chicory, and breeders are attempting to produce cultivars for medicinal purposes. In the modern breeding of chicory important feature is the ability to propagate in vitro culture.
The aim of our study was to assess capacity of natural population of wild chicory for plant regeneration from leaf explants. In the first was examined the effect of various concentrations of BA and TDZ on the regeneration of shoots from leaf explants (0.5 cm²). After that, 25 plants were propagated on the medium which was found as optimal. Then, their callus growth and shoots regeneration capacities were compared. The majority of the shoots were regenerated from callus but direct organogenesis was also observed (8%).
Shoot regeneration was found to be the most efficient on MS medium containing 5MµBA and 3MµTDZ –97% of the explants produced shoots, while the average number of shoots was 15.5. The wild chicory shoots rooted easily.

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