GENE EXPRESSION EVALUATION OF DHN GENE IN SOME WHEAT (Triticum asetivum L.) CULTIVARS UNDER ABIOTIC STRESS

Nasr, Ohamed; El-Absawy, Elsayed; Abd El-Aziz, Amal; Salah, Ahmed

doi:10.21608/jpd.2015.42793

GENE EXPRESSION EVALUATION OF DHN GENE IN SOME WHEAT (Triticum asetivum L.) CULTIVARS UNDER ABIOTIC STRESS

Document Type : Original Article

Authors

¹ Molecular Biology Dept., Genetic Engineering and Biotechnology Research Institute, GEBRI, Sadat city, Sadat city University, Egypt.

² Bioinformatics Dept., GEBRI, Sadat city University, Egypt.

10.21608/jpd.2015.42793

Abstract

Drought is one of the major causes of dramatic yield loss in crop plants. Plants perceive and respond to stress. Upon perception of stress, a signal is communicated to downstream components resulting in change of gene expression and thereby of proteins required for the initial damage-repair and physiological re-programming for better adaptation.The relative water content (RWC) used as one of the most reliable indicators for defining both the sensitivity and the tolerance of wheat plants to water deficit.
The real-time quantitative PCR used to monitor the expression pattern of dehydrin gene in Triticum asetivum leaves under drought and ABA stress. The results showed a high quantitative up-regulation of dehydrin, Identifying the high tolerant varieties Vorobey and Giza 168 exhibited a higher activation of DHN and at the same time a high relative expression level of the gene. Similar gene expression profile was observed in Sakha 93. However, relative expression level was lower for Finisi, Gemmiza 7and Gemmiza 9 compared to other varieties.

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