Abo Shama, H., Ghareeb, H. (2015). DEVELOPMENT OF HAPLOID PLANTS THROUGH ANTHER CULTURE TECHNIQUE IN WHEAT ( Triticum aestivum L. ). Journal of Productivity and Development, 20(3), 285-296. doi: 10.21608/jpd.2015.42802
Haron Abo Shama; Hala Ghareeb. "DEVELOPMENT OF HAPLOID PLANTS THROUGH ANTHER CULTURE TECHNIQUE IN WHEAT ( Triticum aestivum L. )". Journal of Productivity and Development, 20, 3, 2015, 285-296. doi: 10.21608/jpd.2015.42802
Abo Shama, H., Ghareeb, H. (2015). 'DEVELOPMENT OF HAPLOID PLANTS THROUGH ANTHER CULTURE TECHNIQUE IN WHEAT ( Triticum aestivum L. )', Journal of Productivity and Development, 20(3), pp. 285-296. doi: 10.21608/jpd.2015.42802
Abo Shama, H., Ghareeb, H. DEVELOPMENT OF HAPLOID PLANTS THROUGH ANTHER CULTURE TECHNIQUE IN WHEAT ( Triticum aestivum L. ). Journal of Productivity and Development, 2015; 20(3): 285-296. doi: 10.21608/jpd.2015.42802
DEVELOPMENT OF HAPLOID PLANTS THROUGH ANTHER CULTURE TECHNIQUE IN WHEAT ( Triticum aestivum L. )
Plant Biotech Dept . Genet. Engin. and Biotech .Res. Inst ( GEBRI), Sadat City, University Menufiya, Egypt.
Abstract
Anther culture was utilized to develop haploid plants in wheat .This study was carried out at Plant Biotechnology Department, Genetic Engineering and Biotechnology Research Institute ( G E B R I ) from 2009 to 2014 years . Three varieties of wheat were used , Sids1, Gemmeiza 9 and Giza 168. The studied factors were, cold and chemical pretreatments, growth regulators concentrations of 2,4-D and kinetin and effect of source of spikes . The results indicated that the cold pretreatment had no effect on anther response of three wheat varieties, the chemical pretreatments by sterilization substances (ethanol alcohol 70 % or mercurec chloride 1g /L or thiourea 2 g /L ) were very important and essential to induce callus from anther . The three treatments of ( 1mg /L 2,4-D ) , ( 2 mg /L 2,4-D ) and ( 2 mg /L 2,4-D + 0.1 mg /L kinetin ) gave the highest response of callus induction and plant regeneration . Androgenesis improved with increasing the cultured area which the spikes were collected from it .
View on SCiNiTO
Top