Anther culture was utilized to develop haploid plants in wheat .This study was carried out at Plant Biotechnology Department, Genetic Engineering and Biotechnology Research Institute ( G E B R I ) from 2009 to 2014 years . Three varieties of wheat were used , Sids1, Gemmeiza 9 and Giza 168. The studied factors were, cold and chemical pretreatments, growth regulators concentrations of 2,4-D and kinetin and effect of source of spikes . The results indicated that the cold pretreatment had no effect on anther response of three wheat varieties, the chemical pretreatments by sterilization substances (ethanol alcohol 70 % or mercurec chloride 1g /L or thiourea 2 g /L ) were very important and essential to induce callus from anther . The three treatments of ( 1mg /L 2,4-D ) , ( 2 mg /L 2,4-D ) and ( 2 mg /L 2,4-D + 0.1 mg /L kinetin ) gave the highest response of callus induction and plant regeneration . Androgenesis improved with increasing the cultured area which the spikes were collected from it .
Abo Shama, H., & Ghareeb, H. (2015). DEVELOPMENT OF HAPLOID PLANTS THROUGH ANTHER CULTURE TECHNIQUE IN WHEAT ( Triticum aestivum L. ). Journal of Productivity and Development, 20(3), 285-296. doi: 10.21608/jpd.2015.42802
MLA
Haron Abo Shama; Hala Ghareeb. "DEVELOPMENT OF HAPLOID PLANTS THROUGH ANTHER CULTURE TECHNIQUE IN WHEAT ( Triticum aestivum L. )", Journal of Productivity and Development, 20, 3, 2015, 285-296. doi: 10.21608/jpd.2015.42802
HARVARD
Abo Shama, H., Ghareeb, H. (2015). 'DEVELOPMENT OF HAPLOID PLANTS THROUGH ANTHER CULTURE TECHNIQUE IN WHEAT ( Triticum aestivum L. )', Journal of Productivity and Development, 20(3), pp. 285-296. doi: 10.21608/jpd.2015.42802
VANCOUVER
Abo Shama, H., Ghareeb, H. DEVELOPMENT OF HAPLOID PLANTS THROUGH ANTHER CULTURE TECHNIQUE IN WHEAT ( Triticum aestivum L. ). Journal of Productivity and Development, 2015; 20(3): 285-296. doi: 10.21608/jpd.2015.42802
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