DEVELOPMENT OF HAPLOID PLANTS THROUGH OVARY CULTURE TECHNIQUE IN WHEAT ( Triticum aestivum L ).

Document Type : Original Article

Authors

1 Plant Biotech. Department Genetic Engineering and Biotech. Research Institute ( GEBRI ), Sadat City. Univ. Menufyia, Egypt

2 Plant Biotech. Department Genetic Engineering and Biotech. Research Institute ( GEBRI ), Sadat City. Univ. Menufyia, Egypt.

Abstract

This research was done at department of Plant Biotechnology, Genetic Engineering and Biotechnology Research Institute( GEBRI ), Sadat City university, Menufyia, Egypt from 2009 to 2014 years to study the effect of four factors affected  callus induction of ovary cultur i.e.(cold pretreatment, chemical pretreatments, growth regulators and spikes sources. and establishment a technique to produce haploid plants. Three wheat varieties were used, Sids 1, Gemmeiza 9 and Giza 168. The results indicated that, chemical pretreatments by sterilization substances (Ethanol alcohol 70% or Mercurec chloride 1g/L or Thiourea 2g/L) were very important and essential to induce calli from ovaries. The combination between (1 and 2 mg /L 2,4-D ) and (0 , 0.1 and 0.2 mg/L kinetien) gave the highest response of callus induction and plant regeneration percentages. The estimates were increased and developed with increasing  the cultured area which the spiks were collected from it. The estimates were varied with varieties.
 

Keywords

Journal of Productivity and Development
Volume 20, Issue 3
August 2015
Pages 297-306

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