ElSayed, E., El-Hamady, M. (2007). RESPONSE OF COWPEA PLANTS (Vigna unguiculata (L.) WALP.) TO SOME BIOSTIMULANTS AND ORGANIC NUTRIENTS DURING LATE SUMMER SEASON.. Journal of Productivity and Development, 12(1), 237-250. doi: 10.21608/jpd.2007.44954
El-Saied ElSayed; Mahmoud El-Hamady. "RESPONSE OF COWPEA PLANTS (Vigna unguiculata (L.) WALP.) TO SOME BIOSTIMULANTS AND ORGANIC NUTRIENTS DURING LATE SUMMER SEASON.". Journal of Productivity and Development, 12, 1, 2007, 237-250. doi: 10.21608/jpd.2007.44954
ElSayed, E., El-Hamady, M. (2007). 'RESPONSE OF COWPEA PLANTS (Vigna unguiculata (L.) WALP.) TO SOME BIOSTIMULANTS AND ORGANIC NUTRIENTS DURING LATE SUMMER SEASON.', Journal of Productivity and Development, 12(1), pp. 237-250. doi: 10.21608/jpd.2007.44954
ElSayed, E., El-Hamady, M. RESPONSE OF COWPEA PLANTS (Vigna unguiculata (L.) WALP.) TO SOME BIOSTIMULANTS AND ORGANIC NUTRIENTS DURING LATE SUMMER SEASON.. Journal of Productivity and Development, 2007; 12(1): 237-250. doi: 10.21608/jpd.2007.44954
RESPONSE OF COWPEA PLANTS (Vigna unguiculata (L.) WALP.) TO SOME BIOSTIMULANTS AND ORGANIC NUTRIENTS DURING LATE SUMMER SEASON.
1Vegetable Department, Horticulture. Research Institute, Agriculture Research Center, Cairo, Egypt.
2Plant Production Department, Efficient Productivity Institute., Zagazig University, Egypt.
Abstract
Two field experiments were conducted during 2005 and 2006 at El-Baramon Experimental Farm, El-Dakahllia Governorate, Hort. Research Institute. In order to study response of cowpea plants El-Balady cv. grown during late summer season to foliar feeding with some biostimulants (amino acids– seaweed extracts) and organic nutrients,(potassium borate citrate, organic boron,zinc citrate, calcium citrate, NPK-Humate)besides to NAA, on growth, mineral composition and yield and its components towards, maximizing its productivity under such season condition. The results could be summarized as follows: The applied treatments were significantly differed among them and were superior the control in all growth parameters, mineral concentration (NPK and Ca) in leaves, seed yield and its components during the two seasons. The best ones in diminished order were KB-citrate NPK-humate, NAA, amino acids, B-EA (organic boron), seaweed extracts, Zn-citrate and at least Ca-citrate, those increased their plants, dry weight and total seed yield increased over the control by (35.67%,43.9%), (26.79% ,35.01%), (26.36% ,35.1%), (24.66% ,30.8%), (24.63% ,32.3%), (17.98% ,27.5%) ,(16.64%,25.8%) and (5.73%,20.5%) means of the two seasons, respectively. Also, these treatments resulted in significant increase in concentration of N,P,K and Ca in leaves compared with the control one. It could be sprayed late summer grown cowpea plants with potassium borate citrate (3ml/L) or NPK-humate (2.5ml/L) to obtain the best growth and mineral concentration and the maximum total seed yield.
RESPONSE OF COWPEA PLANTS (Vigna unguiculata (L.) WALP.) TO SOME BIOSTIMULANTS AND ORGANIC NUTRIENTS DURING LATE SUMMER SEASON.
El-S. L. El-S. Fathy, *and M. M. El-Hamady**
*Vegetable Department, Horticulture. Research Institute, Agriculture Research Center, Cairo, Egypt.
** Plant Production Department, Efficient Productivity Institute., Zagazig University, Egypt.
ABSTRACT
Two field experiments were conducted during 2005 and 2006 at El-Baramon Experimental Farm, El-Dakahllia Governorate, Hort. Research Institute. In order to study response of cowpea plants El-Balady cv. grown during late summer season to foliar feeding with some biostimulants (amino acids– seaweed extracts) and organic nutrients,(potassium borate citrate, organic boron,zinc citrate, calcium citrate, NPK-Humate)besides to NAA, on growth, mineral composition and yield and its components towards, maximizing its productivity under such season condition.
The results could be summarized as follows:
The applied treatments were significantly differed among them and were superior the control in all growth parameters, mineral concentration (NPK and Ca) in leaves, seed yield and its components during the two seasons.
The best ones in diminished order were KB-citrate NPK-humate, NAA, amino acids, B-EA (organic boron), seaweed extracts, Zn-citrate and at least Ca-citrate, those increased their plants, dry weight and total seed yield increased over the control by (35.67%,43.9%), (26.79% ,35.01%), (26.36% ,35.1%), (24.66% ,30.8%), (24.63% ,32.3%), (17.98% ,27.5%) ,(16.64%,25.8%) and (5.73%,20.5%) means of the two seasons, respectively. Also, these treatments resulted in significant increase in concentration of N,P,K and Ca in leaves compared with the control one.
It could be sprayed late summer grown cowpea plants with potassium borate citrate (3ml/L) or NPK-humate (2.5ml/L) to obtain the best growth and mineral concentration and the maximum total seed yield.
In recent years, stressful high temperature prevails in Egypt during late summer season. This frequently cause severe depression in different performances of vegetable crops including cowpea (Wein et al., 1993; Fathy and Farid, 2000; Fathy and Khedr, 2005 b).
At the physiological and metabolical basis high temperature induces serious internal disordered case and drastic events of bioassimilates, hormones and nutrients shortage and imbalances and lacking of their supply into reproductive sinks; as well as induces serious injurious oxidative stress. This case lead to serious agronomical problems of poor fruit setting, abscission of fruits and low productivity (Wien and Zhang, 1991, Huberman et al., 1997; Fathy and Farid, 2000, Khedr et al., 2004).
In addition, thermotolerance and thermoinjury known to be associated with the internal ions level that play a role in the activation of the transporter enzymatic system (H-ATP-ase) membrane pump and certain H+, K+ and Ca++ balance leading to stress protection processes and responses (Palta, 1990).
Also, with the capability to activate the antioxidantal defensive enzymatic systems against high temperature inducable degradable oxidative stress (Dicknson et al., 1991 and Cakmak and Marschner, 1992).
Based on the above mentioned information and the cited literature, the present work aimed to ameliorate the propable destructive effects of high temperature condition, restore the plants into normal function and enhanced productivity case. This suggested to be achieved by application and then identification the effect of promising natural biostimulants and organic nutrients, i.e. amino acids, seaweed extracts, NPK- humate complex, K-B citrate, Ca-citrate, Zn-citrate, and B-EA (boron organocomplex) as well as NAA on different performance of cowpea plants.
Herein of value to cited the relevant information about these treatments Franco et al. (1998) showed that foliar spraying of amino acids improved fruit yield and quality of tomato. Fathy and Khedr (2005a) illustrated that the frequent foliar feeding with amino acids, Zn-citrate and phytoextract (yeast) individually or in combination was considerably recovered the biotic stressed tomatoes due to their beneficial effects on the metabolic potential for synthesis of amino acids, protein, sugars, carbohydrates also to their antioxidant defensive function including synthesis of carotenoids and phenols also enhancing efficient on uptake of N, P and K and at least corresponding these responses with normal growth and high fruit yielding capacity.
Many different effects have been reported as a result of the application of seaweed extracts, including enhanced crop yield, increased uptake of inorganic nutrients from the soil, improved different growth aspects, induced resistance to stress conditions(biotic and a biotic ones). Such extracts know to exhibit different regulatory, and defensive effects through elicitation and singling of different physiological and metabolical processes (Metting, 1990; Blunden, 1991). Whapham et al. (1993) used alkaline extract of Ascophyllumnodsum either as foliar or soil application, this result in improving growth and chlorophyll content of treated plants El-Aidy et al. (2002) found that, foliar spraying of different commercial formulations of seaweed extracts (Maxicrop and ASCO) considerably improved growth, N and P content of leaves, early and total yield of sweet pepper. Similar results were obtained by using foliar feeding of brown algae (Ascophyllum nodsum) (Goemar) (Klarzynski et al., 2006 and Zdravkovic et al., 2006) on bean.
Meanwhile it was demonstrated that, humic acid, alone and/or its, formulations in form of mineral organocomplex applied through foliar or root feeding resulted in considerable improvement in different crops growth, mineral contents and bioconstituents and productivity.
On the other hand, it was well known that, some nutrients (K, Ca, Zn and B) specially in organic chelated form are efficiently participated in the internal processes associated with protection against high temperature stress condition also, that their foliar spraying is considerably improved different performances of the stressed plants (Franco et al., 1998; Wenyi et al., 1999; Khedr et al., 2004 ; Fathy and Khedr, 2005 a and b).
In addition, it was found that foliar application of NAA especially during high temperature condition is used as a standard practice for reducing abscission of reproductive organs, improving fruit set and productivity also enhancing growth and mineral content of different crops (Pressman et al., 1998 with pepper; Singh and Awasthi, 1998 with Vigna radiate; Resmi and Gopalakrishnan, 2004 with Vigna unguiculata ( L.) Walp .
Therefore, the aim of the present study to investigate the response of cowpea plants (Vigna unguiculata (L.) Walp.) to some biostimulants and organic nutrients during late summer season.
MATERIALS AND METHODS
Two field experiments were performed at El-Baramon Experimental Farm, El-Dakahllia governorate, Horticulture Research Institute during two summer successive seasons of 2005 and 2006 on cowpea plants. This work aimed to study the effect of some biostimulants and organic nutrients on different performances of this crops under high temperature stressful conditions of late summer season towards maximizing its productivity under such condition.
May,2nd seeds of cowpea El-Balady cv. were sown in hills on one side ridge of 5m long and 0.6m wide and 0.25m between hills. After emergence the plants thinned leaving one plant per hill. The plot area was 9m2with planting density of about 26600plant /fed.
These plants were sprayed 3 times (20 day after sowing and again every 20 day) with the solution of the following treatment :-
1-Amino acids (35.0% w/v total amino acids) from biological origin, commercially named Mëgfol introduced from Valagro Comp., Italy., registered in Egypt as biostimulants with number 4827, applied at concentration of 1.5ml/L.
2-Seaweeds, natural phytoextract of Ascophyllum nodosum a brown algae introduced from Goëmar Laboratoires, France, registered in Egypt as biostimulant (Goëmar BM86) with No. 5468. It contains more than 17 essential amino acids (5738µ mol / L), vitamins (40 mg / kg) phytohormons (200 mg / L), Betaines (140 mg / L), polysaccharides (50% DM), also enreached with N, Mg, S, B and Mo, applied at concentration of 2.5ml/L.
3-NPK-Humate: 10% humic acids + 8% (N) + 8%( P2O5) + 8%( K20) registered with NO. 5709 Horticulture. Research Institute, applied at concentration of 2.5ml/L.
4-Potassium borate citrate (35% K20 , 5% B), formulation of Hort. Res. Inst. applied at concentration of 3.0ml/L.
5-Calcium citrate (Ca-cit.) 25% Ca, registered No. 5713 Hort. Res Inst., applied at concentration of 5ml/L.
6-Zinc citrate (Zn cit.) 12% Zn, registered No. 5714, Hort. Res Inst., applied at concentration of 1.5ml/L.
7-Organic boron (B-EA) (Solbor) 8% B, from Hort. Res Inst., applied at concentration of 1ml/L.
8-NAA, from Alfrut commercial formulation (3.5% pure NAA w/v), from SIFO, Italy applied at concentration of 10mg/L.
9-Control, only sprayed with water.
All cultural practices were performed as recommended by Hort. Res Inst.
Randomized complete block design was adopted and the treatments were replicated three times.
At 75 days from sowing 5 plants from each plot were taken and No. of leaves, No. of shoots, fresh and dry weight (gm) were recorded and calculated per plant. Samples of fully expanded mature leaves were taken from each plot 10 days after the last spray for chemical analysis of N, P, K and Calcium. Nitrogen, P and K were analyzed and determined according to methods of Horneck and Miller (1998), Sandell (1950) and Horneck and Hanson (1998), respectively. Calcium was analyzed using atomic absorption method.
Yield and its components: number of pods/plant and number of seeds/pod were recorded from the cumulative harvestings of ten marked plants of each plot. The dried seeds weight determined on plot basis then, seed yield (gm)/plant and total seed yield (ton)/fed. were calculated.
All data were statistically analyzed according to (Snedecor and Cochran ,1980).
Also, data of air temperature(Co) and relative humidity(%) as well as of soil analysis during 2005 and 2006 are presented herein in Tables 1 and 2.
Table (1): Means monthly air temperature(Co) and relative humidity (%) at El-Dakahlia Governorate, during 2005 and 2006.
Months
Air temperature (Co)
Relative Humidity
(%)
2005
2006
Max.
Min.
Max.
Min.
2005
2006
May
30.37
15.95
29.52
15.13
61.10
61.81
June
31.66
19.87
32.85
19.80
65.67
65.97
July
33.55
20.30
32.69
21.61
69.87
70.55
August
35.68
24.39
34.47
22.55
71.00
71.26
September
33.45
20.75
33.65
19.82
68.10
65.67
Source: Mansoura Meteorological Station.
Table 2 : Mechanical and chemical analysis of the experimental soil during 2005 and 2006.
Soil properties
2005
2006
Clay (%)
59.95
58.62
Silt (%)
24.94
26.50
Sand (%)
15.11
14.88
Texture
Clayey
Clayey
Organic matter % (O.M.,%)
1.95
1.89
Ca CO3 (%)
1.99
2.35
Available N ( ppm)
21.12
22.2
Available P ( ppm)
11.72
9.36
Available K ( ppm)
391
399
EC m. mhos/cm (25oC)
1.50
1.48
pH
7.9
8.1
The experimental soil was analyzed by using standard method described by Page (1982).
RESULTS AND DISCUSSION
1- Vegetative Growth:
The data in Table 3 prove that, all the applied foliar sprays of biostimulants and minerals in organic form were significantly increased number of leaves and shoots, fresh and dry weight of their plants in comparison with the control one during the two seasons. The most stimulatory and potent effects in diminished order were of KB-citrate followed by NPK-humate and NAA, then B-EA and amino acids, Zn-citrate and seaweed extracts and at least Ca-citrate respectively.
These treatments increased dry weight of plants relative to the control one by 35.67%, 26.79%, 26.36%, 24.66%, 24.63%, 17.98%, 16.64%, and 5.73% (mean value of the two seasons), respectively.
These results were inconsistent with those obtained by Wenyi et al. (1999); Fathy et al. (2003) and Khedr et al.( 2004) for foliar feeding with K, Ca, Zn, and B under stress condition; Neri et al., 2005, Padem et al., 2007, El-Aidy et al., 2002 (humic acids and seaweed extracts), Franco et al., 1998 (amino acids); Resmi and Gopalakrishnan, 2004 (NAA). Seemingly the applied treatments achieved growth increments in dry weight ranged from 16.6 to 35.6%, except that of Ca-citrate 5.7%, also, some treatments such as KB-cit. were superior NAA (the standard synthetic phytoregulator) or some what equal it NPK-humate, B-EA and amino acids. This to far extent proved high stimulatory effects for these treatments).
Herein, the lowest growth behavior of untreated cowpea plants proved that, these plants, were to certain extent stressed by high temperature. This could be due to
the inhibitory effect of high temp. condition on uptake and in turn concentration of N, P, K and Ca in control plant leaves (Table 4). Also may be due to the inducablestress related internal disturbances and shortage of bioconstituents, hormones and nutrients (Wien and Zhang, 1991 and Huberman et al., 1997). High temperature known to induce serious physiological oxidative stress of elevated toxic level of ROS, thereby more destructive and degradable effects on the whole biosynthetic machinery (Dicknson et al., 1991 and Cakmak and Marschner, 1992). So it is not superising to hold great responses and mutigation for the treated cowpea plants under present work climatic condition (Table 2).
The most growth encourages effect of KB-citrate may be due to that this formulation contain K and B of the similar and harmonic synergetic action, complexes with organic moiety of citrate, ensuring potent K and B nutrition for more enhancement of carbohydrates, proteins, enzymes, phenols and energy synthesis and saving as well as more osmoregulation and protection, lignification and stress protection. At the same basis the beneficial effect of B organo complex B-EA may be explained (Marschner, 1995, Khedr et al., 2004 and Fathy et al., 2003). The organo / mineral complex of NPK-humate is greatly induced high balanced N, P, K and Ca concentration within cowpea leaves (Table 4), besides to the known encouragous action of humic acids, for protein synthesis, nutrients translocation, antioxidantal enzymes, root proliferation and foliar growth (Sun et al., 2004 and Trkmen et al., 2005).
NAA as a commonly used synthetic auxin, known to stimulate cell division and differentiation process of root and tip, activate oxidative stress protective peroxidases (Hess, 1981).
Amino acids and seaweeds extracts via their amino acids and other constituents enhanced biosynthesis of protein / enzymes (antioxidantal and transporter ones) and/or compensated it under stress condition, improve the capacity of photosynthesis, carbohydrate and phenol content as well as the biochemical defensive responses against environmental stresses (Franco et al., 1998, Fathy and Khedr, (2005a)on amino acids,(Zdravkovic et al., 2006, Klarzynski et al., 2006) on seaweed extracts.
Lastly, the beneficial influence of foliar applied organometal complexes of Zn and Ca (citrate form) this form suggested to be due to their growth related efficient involvements and utilization, i.e. cell division, hormone synthesis and balance, stress signaling and regulatory roles (Marschner, 1995).
2- Mineral Composition:
From data presented in Table 4, it was recognized that, all foliar spray treatments significantly differed among them and were highly superiored the control in concentration of N, P, K and Ca in leaves during the two seasons. The same data cleared that the highest N and P concentration recorded with NPK-humate followed by KB-citrate and seaweed extracts. Where as, the highest K concentration was of KB-citrate followed by NPK-humate, NAA and B-EA treatments. Ca-citrate followed by KB-citrate and NAA showed the considerable highest Ca in leaves of their plants. The results, were in the same trends in both seasons.
Treatments
N%
P%
K%
Ca%
2005
2006
2005
2006
2005
2006
2005
2006
Amino acids
3.53
3.59
0.261
0.253
3.08
3.01
2.30
2.28
Seaweed extracts
3.43
3.49
0.264
0.261
2.90
2.84
2.21
2.14
NPK-Humate
3.75
3.93
0.342
0.337
3.50
3.51
2.31
2.31
KB-cit.
3.50
3.60
0.326
0.321
3.93
3.84
2.42
2.39
Ca-cit.
3.05
3.17
0.243
0.242
2.40
2.45
2.91
2.75
Zn-cit.
3.07
3.17
0.263
0.258
2.85
2.79
2.22
2.12
B-EA
3.35
3.44
0.259
0.248
3.20
3.16
2.18
2.09
NAA
3.27
3.47
0.295
0.282
3.26
3.26
2.40
2.33
Control
2.69
2.88
0.243
0.233
2.75
2.68
2.06
2.00
L.S.D (0.05)
0.08
0.05
0.006
0.004
0.03
0.10
0.08
0.07
Table 4: Effect of some biostimulants and organic nutrients on mineral composition of cowpea leaves during2005 and 2006.
The present significant increments in mineral concentration values of all treatments over the control may be explained based on the degradable effect of high temperature condition (Table 1) and its paralleled inducible oxidative stress on plasma membrane and its binding transporter enzymes and then the consequential inhibition for, mineral, uptake and translocation processes (Pallta, 1990 and Dicknson et al.,1991).
Meanwhile, the direct or indirect involvementof the applied treatments, either by feeding with certain minerals or to protect and recovered specific transporter enzymes and/or the whole metabolic machinery under such conditions is decumentated (Palta, 1990 and Marschner, 1995) on K, Ca, Zn; (Klanzynski et al., 2006) on seaweed, ex.; (Fathy and Khedr, 2005a) on amino acids and Zn-citrate; (Neri et al., 2005, Padem et al., 2007) on humic acids, (Singh and Awasthi, 1998; Resmi and Gopalakrishnan, 2004) on NAA.
3- Yield and its Components:
From the data illustrated in Table 5, it was shown that, all treatments were greatly increased yield and its components over the control in the two seasons and differed among them in most cases. The significant highest total seed yield/fed. was of KB-citrate treatment, its increment reach 43.9% over the control, this considerably was due to the superiority of this treatment in number of seeds/pod and seed yield/plant but not in number of pods/plant, during the two seasons.
Also due to its noticable enhancable effect on the accumulation of dry matter and concentration of minerals in their plants foliage (Tables 3 and 4). So the seed yield enhancement of this treatment suggested to be mainly via the synergetic efficient role of its K and B together in photoassimilate formation and transportation into the reproductive sinks, in turn increasing-yield mass, besides to their other known regulatory and protective functions (Marschner, 1995). Also in similar to results of
(Fathy et al., 2003 and Khedr et al., 2004). The same data proved that, the subsequent effective treatments were, NAA and NPK-humate those which equally increased total yield of seeds by 35.0% and greatly improved all yield components specially the number of pods/plants with less or no differences between them in most cases.
This was in similar to the findings obtained by Samet (2004), Sun et al., (2004) for humic acids, Singh and Awasthi (1998); Resmi and Gopalakrishnan (2004) for NAA.
It was also obvious that the significantly following seed yield encouragous treatments were B-EA, amino acids, seaweed extracts, Zn-citrate and at least Ca-citrate. Those increased total seed yield of cowpea plants over the control by 32.3%, 30.8%, 27.5%, 25.8% and 20.0% (mean of the two seasons) respectively. This in the same line was due to their enhancable effects either on number or mass of the reproductive organs as shown by their effect on number of seeds / pod, number of pods/plant and seed weight/plant in both seasons. Also, due to their advatagous effect on growth and mineral / concentration of their plants in similar fashion. (Tables 3 and 4).
These results were in agreement with those obtained by Wenyi et al., 1999; Plese et al., 1998; Khedr et al., 2004 for Zn, B and Ca; Franco et al., 1998 for amino acids; Blunden et al., 1991 for seaweed extracts.
Conclusively, of interest to be considered and give more attention for the following point :
1-The essentiality of foliar feeding with certain one or more elements of similar and synergistic roles such as K and B in metal-organo complex from to exert satisfactory nutritional, regulatory and stress defensive effects .
2-Using minerals / humic acids complex as new and advanced nutrition tool.
3-Under high temperature condition cowpea plants is exhibit certain sensitivity level and in turn it required some encouragous treatments for mutigation and maximizing its productivity .
REFERENCES
Blunden, G. (1991): "Agricultural Uses of Seaweeds and Seaweed Products". In: European seaweed resources: uses and Potential, PP 65-81.1991 J. Wiley & Sons, Chichester, England.
Cakmak, T. and H. Marschner (1992). Magnesium deficiency and high light intensity enhance activities of superoxide dismutase, ascorbit peroxidase and glutathione verductase in bean leaves. Plant physiol., 98 : 1222 – 1227.
Dicknson. C. D.; T. Altabella, and M. J. Chrispeels, (1991): Slow growth phenotype of transgenic tomato expressing oplastic invertaste. Plant Physiol.,95: 420 – 425.
El-Aidy, F.; A. I. El-Zawily; B. I. El-Sawy and E. M. Hamed (2002). Effect of seaweed extracts on sweat pepper plant grown under protected cultivation 2nd Inter. Conf. Hort. Sci. 10 – 12 Sept. Kafr El-Sheikh, Tanta Univ., Egypt.
Fathy El-S. L. El-S.; A. M. M. Abd-Rahman and Z. M. A. Khder, (2003). Response of broad bean to foliar spray of different k-sources and energy related organic compounds (EROC) to induce better internal K and sugars case towards better growth and productivity. J. Agric. Sci. MansouraUniv.,28 (4) : 2935 – 2954.
Fathy, El-S. L. El-S. and S. Farid, (2000). Effect of some chemical treatments, yeast preparation and royal jelly on some vegetable crops grown in late summer to induce their ability towards better thermal tolerance. J. Agric. Sci. Mansoura Univ.,25 (4): 2215 – 2246.
Fathy El-S. L. El-S. and Z. M. A. Khedr (2005 a). Program and new treatments for reducing the infection severity and inducing tolerability of tomato yellow leaf curt virus (TYLCV) in fall season. Proceeding of the 6th Arabian conference for Horticulture. PP: 221 – 245, Suez Canal University, Ismalia, Egypt.
Fathy, El-S. L. El-S. and Z. M. A. Khedr (2005 b). Maximizing sweet pepper productivity during late summer using different calcium sources and zinc levels. Proceeding of the 6th Arabian conference for Horticulture, PP 519 – 533. Suez Canal Univ. Ismalia, Egypt.
Franco, J. A ; P.J. Perez-Saura, and A. Duran (1998). Calcium for correcting blossom-end rot in tomato Horticulture, Revista de Hortalizas, flores, plantas ornamentales, Y Viveros, No. 126, 90-92.
Hess, O. (1981). Plant Physiology: molecular, biochemical and physiological fundamental of metabolism and development P.P: 197-200, Narosa Publ. H. 2/35 Ansari Road New Delhi-110002, India.
Horneck, D. A. and D. Hanson, (1998). Determination of K and Na by flame emission spectrophotometery. In: Handbook of Ref. Meth. for plant analysis, Kalra, Y.P. (ed.): 153 -155
Horneck, D.A. and R.O. Miller (1998). Determination of total N in plant tissue. In Handbook of Ref. Meth. of Plant Analysis. Kalra, Y.P. ed., 75 – 83.
Huberman, M.; J. Riov ; B. Alon and R. Goren (1997). Role of ethylene biosynthesis and auxin content and transport in high temperature-induce abscission of pepper reproductive organs. J. Plant Growth Regulation,16 (3): 129 135.
Khedr, Z. M. A.; El-S.L. El-S. Fathy, and A.M.Moghazy (2004). Effect of some nutrients and growth substance on productivity of eggplant (Solanum melongena var esculenta) growing under high temperature conditions. Annals of Agric.Sc.,Moshtohor ,Vol..42 (2):583-602.
Klarzynski, O.; F. Elodie ; Z. Mickael and J. Jean-Marie(2006). Alga filtrates. Scientific basis for their effects on plant physiology. Phytoma (10); plant defence, N.597, Lab. Go., Av. du General – Potton 354/8 Saint Malo Cedex, France.
Marchner, H. (1995). Mineral Nutrition of Higher Plants, Academic press, Harcourt Brac Jouanovish, publishers P. 674.
Metting, B. (1990):"Agronomic Uses of Seaweed and Microalgae". Introduction to applied phycology, P.P 589-627 1990 SPB Academic Publishing by: The Hague, NL.
Neri D.; E. M Lodolini; K. Muthuchelian, G. Bonanomi and, F. Zucconi (2005) Physiological responses to several organic compounds applied to primary leaves of cowpea (Vigna sinensis L.) ISHS Acta Hort. 594: International Symposium on Foliar Nutrition of Perennial Fruit Plants.
Padem, H; A. Ocal and R. Alan (2007). Effect of humic acid added to foliar fertilizer on quality and nutrient content of eggplant and pepper seedlings. Symposium Greenhouse Management for Better Yield & Quality in Mild Winter Climates.
Page, A.L. (1982).Methods of Soil Analysis. Part 2. Chemical and Microbiological Properties 2nd Ed. Agron. J. Amer. Soc. Agron., Inc. Publ. Madison, Wis. U.S.A.
Palta, T. P. (1990). Stress interactions at the cellular and membrane levels. Hort Science, 25 (11): 1377 – 1381.
Plese, L. P. M.; C. S. Tiritan; E. I. Yassuda, L. I. Prochnow, J.E.Corrente and S. C. Mello (1998). Effects of application of calcium and boron on occurrence of blossom-end rot and on yield of tomato grown in the greenhouse. Scientia Agricola, 55, 1, 144 – 148, 10 ref.
Pressman, E.; K. Rosenfeld; R. Shaked; H. Moshkovitz; B. Aloni; E. Tomer and M. Coher (1998). Histological examination of low temperatures, TIBA – induced swelling of pepper ovaries. Bioch. and Life Sci., 25, 3.
Resmi R. and Gopalakrishnan (2004). Effect of plant growth regulators on the performances of yard long bean (Vigna unguiculata var. sesquipedalis (d.) verdcourt). Journal of Tropical Agriculture, 42 (1-2): 55-57.
Samet, H. (2004). The effect of Mn applied to soil and leaf a long with FYM and humic acid on the content of protein, v.c. and some yield parameters in pepper. P.H.D. Thesis, Dep. of soil Sci., Ankara Univ.
Sandell, R. (1950).Colorimetric Determination of Traces of Metal. 2nd Ed. Interscience Pub., Inc. New York.
Singh, A. B. and C. P. Awasthi (1998). Effect of growth stimulators on activity of oxidative enzymes in the leaves and status of biochemical constituents in dry mature seeds of green gram (Vigna radiate L. Wilczek). Legume Research, 1998, 21, 3/4, 144 – 150, 19 ref.
Snedecor,G.W.,and W.G.Cochran (1980).Statistical Methods.7th ed.Iowa state Univ. Press., Iowa, U.S.A.
Sun Z.; S. Xue; W. Liang and Y. Liu (2004). Effects of different application rates of humic acid compound fertilizer on pepper and its mechanism of antisenility and incremental yield. 1:Ying Yong Sheng Tai Xue Bao. Jan; 15 (1): 81 – 84.
Trkmen, N; D. Semra; E. Suat and D. Atilla (2005). Effect of arbuscular mycorrhizal fungus and humic acid on the seedling development and nutrient content of pepper grown under saline soil conditions. Doaj Directory of Open Access Journals, Vol.: (5) – Issue: 5.
Wenyi W.; Q. Zhao; S. Jiang and S. Qin (1999). Investigation into factors resulting in non-formation of pods in spring soybeans and its correction .Zhejiang Nongye Kexue , No.1,25-26.
Whapham C. et al., (1993). "Significance of betaines in the increased chlorophyll content of plants treated with seaweed extract". Journal of Applied Phycology, 5:231-234, 1993 Kluwer Academic Publishers Belgium.
Wien, H. C. and Y. Zhang, (1991). Preventation of flower abscission in bell pepper. J. Amer Soc. Hort. Sci., 116 : 516 – 519.
Zdravkovic, M.; Damjanovic, M. and Corokalo , D. (2006).Effects of several foliar fertilizers on bean yield. ISHS,Acta Hort.,579:II Balkan, Symptom Vegetables and Potatoes.
إستجابة نباتات اللوبیا لبعض المنشطات الحیویة والمغذیات العضویة خلال العروة الصیفیة المتأخرة
السعید لطفی السید * محمود محمد الحمادی **
* قسم بحوث الخضر – معهد بحوث البساتین – مرکز البحوث الزراعیة – الجیزة – مصر.
** قسم الإنتاج النباتی (بساتین الخضر) معهد الکفایة الإنتاجیة – جامعة الزقازیق – مصر.
أجریت تجربتان حقلیتان عامی 2005 ، 2006 بالمزرعة البحثیة التابعة لمعهد بحوث البساتین – البرامون دقهلیة لدراسة تأثیر التغذیة الورقیة لبعض المغذیات فی صورة بعض المنشطات الحیویة(الأحماض الأمینیة – مستخلصات الأعشاب البحریة) أو فى صورة عضویة (سترات و بورات ا لبوتاسیوم ، بورون مخلبی ، سترات زنک ، سترات کالسیوم ، هیومات ا لنیتروجین وا لفوسفور وا لبوتاسیوم) إضافة إلى نفثالین حمض الخلیک على النمو والترکیب المعدنی والمحصول ومکوناته لنباتات اللوبیا صنف البلدی تحت ظروف العروة الصیفیة المتأخرة بهدف تعظیم الإنتاجیة تحت تلک الظروف .
وکانت أهم النتائج کما یلی :
اختلفت المعاملات فیما بینها وتفوقت جمیعاً معنویاً على معاملة المقارنة فی تحسین جمیع صفات النمو الخضری تحت الدراسة (عدد الأوراق وعدد الأفرع والوزن الطازج والجاف/ النبات) فی موسمی التجربة. وکانت أفضل المعاملات على التوالی کما یلی :
حیث أدت جمیع هذه المعاملات إلى زیادة الوزن الجاف للنبات بمقدار 35.67% ، 26.79% ، 26.36% ، 24.66% ، 24.63% ، 17.98% ، 16.64% ، 5.73% على التوا لى مقارنة بمعاملة الکنترول .
کما أدت هذه المعاملاتً إلى زیادةمعنویة فى ترکیز النیتروجین والفوسفور والبوتاسیوم والکالسیوم بأوراق نباتات اللوبیا مقارنة بتلک النباتات غیر المعاملة .
فی نفس الوقت اختلفت تلک المعاملات معنویاً فیما بینها وتفوقت على معاملة المقارنة فی التأثیر على المحصول ومکوناته وأدت تلک المعاملات بنفس الترتیب إلى زیادة المحصول الکلی للبذور بمقدار 43.9% ، 35% ، 35.1% ، 30.8% ، 32.3% ، 27.5% ، 25.8% ، 20.5% .
وتوصی الدراسة برش نباتات اللوبیا النامیة خلال العروة الصیفیة المتأخرة بالمغذیات العضویة الآمنة سترات و بورات البوتاسیوم بترکیز (3مل/لتر) أو هیومات ا لنیتروجین والفوسفوروالبوتاسیوم بترکیز (2.5مل/لتر) ثلاث مرات خلال موسم النمو للحصول على أفضل نمو ومحتوى کیماوى وأعلى محصول کلی للبذور .