COMPARATIVE STUDIES ON Catharanthus sp. CULTIVARS USING CHLOROPHYLL CONTENT AND PEROXIDASE ISOZYME

Ibrahim, Ibrahim; Hemeida, Alaa  El Din; .Hussain, Ama; Bekhit, Metwally; Zweil, Amal

doi:10.21608/jpd.2013.42552

COMPARATIVE STUDIES ON Catharanthus sp. CULTIVARS USING CHLOROPHYLL CONTENT AND PEROXIDASE ISOZYME

Document Type : Original Article

Authors

¹ Plant Biotechnology Department, Genetic Engineering and Biotechnology ,Research Institute (GEBRI), Sadat City, Minufiya University , Egypt.

² Bioinformatic Department, Genetic Engineering and Biotechnology, Research Institute Minufiya University, Egypt.

10.21608/jpd.2013.42552

Abstract

Three Egyptian Catharanthus cultivars belong to family apocyanaceae {Roseus, Ocellatus and Albus} as well as calli were derived from leaf explant for each and grown on MS supplemented with 1mg/l NAA and 1 mg/l BA, were studied using chlorophyll contents and peroxidase isozyme . According to the contents of chlorophyll a, b and the total chlorophyll, Roseus, and Ocellatus cultivars' chlorophyll contents were more closely related to each other, but distantly related to Albus cultivar. The total chlorophyll contents were 22.86, 22.08 and 30.51 for Roseus and Ocellatus and Albus cultivars, respectively. Peroxidase isozyme study, exhibited five bands in shoots of the three cultivars, Two bands migrated towards the anode (Pex-A1 and Pex-A2) while the other three bands migrated towards the cathode (Pex-C1, Pex-C2 and Pex-C3). Peroxidase isozyme in the callus samples showed the absence of Pex A1 and Pex A2 bands in Ocellatus cultivar, and the absence of Pex A1, Pex A2 and Pex C3 in Albus cultivar but not in Roseus one where the all 5 bands were exist .No differences between cultivars were resulted when shoot samples.
Conclusively, it could be concluded that chlorophyll contents and isozyme analysis could be considered as a useful tool for cultivar identification because it is reliable, rapid and can provide identification at some stages in plant life cycle. Isozyme technique can be used to identify the desired genotypes and to exclude the cultivars before establishing; it may also serve to identify the more active cultivar, which is essential for biotechnological programmes.

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