EFFECT OF STORAGE AND HEAT ON CHEMICAL-PHYSICAL PROPERTIES AND ANTIMICROBIAL ACTIVITY OF BEE HONEY

Document Type : Original Article

Authors

1 Plant Protection Research Institute, A.R.C., Dokki, Giza – Egypt.

2 Department of Plant Production, Faculty of Technology &Development, Zagazig University, Egypt

3 Plant Protection Research Institute, A.R.C., Dokki, Giza – Egypt

Abstract

Heat treatment and sorage of bee honey caused an increase of the HMF that exceeded greatly the allowance limit (level),meanwhile it decreased the diastase number. Furthermore, the two treatments seemed tobe ineffective on pH, free acidity, lactone and total acidity. The effect of heat and storage on the antibacterial activity of the honey types against 3Gram  negative (Escherichia coli, Enterobacter aerogenes and Klebsiella pneumonia) and 4 Gram positive bacteria (Microcococcus luteu. Enterococcus faecalis, Staphylococcus aureus and Bacillus cereus) was determined using the agar diffusion method.
 The results obtained from the study revealed that, the antimicrobial activity was more pronounced in untreated (heat, storage) honey. Furthermore, the different types of honey (clover, citrus and sugar feeding honey) showed not significant different in antibacterial activity before and after heat application on Egyptian honey samples.
Moreover, the results obtained from the study showed that the inhibitory activity of microbes in honey before exposure to heat is greater than the inhibitory activity after being exposed to heat in a large number of honey samples.
Conclusively, from the previous results it may be recommended not to raise the temperature rise above 28 degrees Celsius during the process of storage.
 
 
 
 

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